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KMID : 0613820120220091166
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Journal of Life Science
2012 Volume.22 No. 9 p.1166 ~ p.1172
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Isolation and Identification of Antioxidant Compounds of Various Solvents Extracted from Eriobotrya japonica Leaves
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Ham Hyeon-Suk
Lee Se-Yeul
Lee Dong-Wan
Seong Jong-Hwan
Kim Han-Soo
Kim Dong-Seob
Lee Young-Guen
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Abstract
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To investigate potential medicinal or functional uses of Eriobotrya japonica, this study focused on the isolation and identification of antioxidant compounds from Eriobotrya japonica leaves. Various solvents were extracted from the leaves, and their scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals was measured, in addition to their superoxide dismutase-like activity, polyphenol compounds, and flavonoid content. Ethyl acetate extract exhibited the strongest scavenging effect in a 0.2 mM solution of DPPH (63.24±2.20%, 81.83±2.10%, and 93.15±2.31% in 0.3, 0.7, and 1.0 mg/ml sample concentrations, respectively). The antioxidant effect of the ethyl acetate extract and methanol extract were generally stronger than that of n-hexane extract. The extracts were further purified by repeated silica gel column chromatography. The antioxidant compounds were identified as phytol, β-sitosterol, and (-)-loliolide using GC/MS.
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KEYWORD
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Eriobotrya japonica leaf, antioxidant, solvent extraction, column chromatography, GC/MS
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